Immunodetection of 5′-methylcytosine on Giemsa-stained chromosomes
نویسندگان
چکیده
منابع مشابه
Immunodetection of 5'-methylcytosine on Giemsa-stained chromosomes.
BioTechniques 527 In higher vertebrates, the major, if not the only, covalent modification of DNA is a methyl-group present at the C5 position of cytosine yielding 5′-methylcytosine (5′-MC). This modification occurs only in the context of CpG dinucleotides, short sequences that are significantly underrepresented in the genome but are found frequently concentrated within so called “CpG island” r...
متن کاملIdentification of Leishmania Species Using PCR Assay on Giemsa-Stained Slides Prepared From Cutaneous Leishmaniasis Patients
BACKGROUND Leishmaniasis is a group of diseases that are created by intracellular parasites of Leishmania. Cutaneous leishmaniasis is considered as one of the health problems in some provinces of Iran. METHODS In this study, a total of 178 Giemsa-stained slides from confirmed cases of cutaneous leishmaniasis were examined. The slides were prepared from the patients with cutaneous leishmaniasi...
متن کاملidentification of leishmania species using pcr assay on giemsa-stained slides prepared from cutaneous leishmaniasis patients.
background: leishmaniasis is a group of diseases that are created by intracellular parasites of leishmania. cutaneous leishmaniasis is considered as one of the health problems in some provinces of iran. methods: in this study, a total of 178 giemsa-stained slides from confirmed cases of cutaneous leishmaniasis were examined. the slides were prepared from the patients with cutaneous leishmaniasi...
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Background Assessment of erythrocytes and leucocytes in thin blood films can be used as an inexpensive diagnostic aid in a series of disease states, e.g. infections, anemia and hematological malignancies. Manual counting of cells is still considered the gold standard for example to establish the level of parasitemia in malaria. However, manual cell counting is time consuming and subject to vari...
متن کاملDiagnosis and Characterization of Leishmania Species in Giemsa-Stained slides by PCR-RFLP
Background: Direct identification of Leishmania species in Giemsa-stained slides without parasite culturing in the areas where multiple species exist, is very helpful. This study was designed to isolate Leishmani spp. from Giemsa-stained smears and to characterize them by PCR technique. Methods: A total of 48 Giemsa-Stained slides from confirmed cases of leishmaniasis were examined under a ligh...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2005
ISSN: 0736-6205,1940-9818
DOI: 10.2144/05384bm01